Observations on the Early Establishment of Foliar Endophytic Fungi in Leaf Discs and Living Leaves of a Model Woody Angiosperm, Populus trichocarpa (Salicaceae).
Identifieur interne : 000D65 ( Main/Exploration ); précédent : 000D64; suivant : 000D66Observations on the Early Establishment of Foliar Endophytic Fungi in Leaf Discs and Living Leaves of a Model Woody Angiosperm, Populus trichocarpa (Salicaceae).
Auteurs : Yu-Ling Huang [États-Unis, Taïwan] ; Naupaka B. Zimmerman [États-Unis] ; A Elizabeth Arnold [États-Unis]Source :
- Journal of fungi (Basel, Switzerland) [ 2309-608X ] ; 2018.
Abstract
Fungal endophytes are diverse and widespread symbionts that occur in the living tissues of all lineages of plants without causing evidence of disease. Culture-based and culture-free studies indicate that they often are abundant in the leaves of woody angiosperms, but only a few studies have visualized endophytic fungi in leaf tissues, and the process through which most endophytes colonize leaves has not been studied thoroughly. We inoculated leaf discs and the living leaves of a model woody angiosperm, Populus trichocarpa, which has endophytes that represent three distantly-related genera (Cladosporium, Penicillium, and Trichoderma). We used scanning electron microscopy and light microscopy to evaluate the timeline and processes by which they colonize leaf tissue. Under laboratory conditions with high humidity, conidia germinated on leaf discs to yield hyphae that grew epiphytically and incidentally entered stomata, but did not grow in a directed fashion toward stomatal openings. No cuticular penetration was observed. The endophytes readily colonized the interiors of leaf discs that were detached from living leaves, and could be visualized within discs with light microscopy. Although they were difficult to visualize within the interior of living leaves following in vivo inoculations, standard methods for isolating foliar endophytes confirmed their presence.
DOI: 10.3390/jof4020058
PubMed: 29772709
PubMed Central: PMC6023450
Affiliations:
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South Campus Drive, Tucson, AZ 85721, USA. ylhuang@nmns.edu.tw.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Plant Sciences, The University of Arizona, 1140 E. South Campus Drive, Tucson, AZ 85721</wicri:regionArea><placeName><region type="state">Arizona</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>National Museum of Natural Science, 1 Guancian Rd., Taichung 40453, Taiwan. ylhuang@nmns.edu.tw.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>National Museum of Natural Science, 1 Guancian Rd., Taichung 40453</wicri:regionArea><wicri:noRegion>Taichung 40453</wicri:noRegion></affiliation></author><author><name sortKey="Zimmerman, Naupaka B" sort="Zimmerman, Naupaka B" uniqKey="Zimmerman N" first="Naupaka B" last="Zimmerman">Naupaka B. Zimmerman</name><affiliation wicri:level="2"><nlm:affiliation>School of Plant Sciences, The University of Arizona, 1140 E. 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South Campus Drive, Tucson, AZ 85721, USA. arnold@ag.arizona.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Plant Sciences, The University of Arizona, 1140 E. South Campus Drive, Tucson, AZ 85721</wicri:regionArea><placeName><region type="state">Arizona</region></placeName></affiliation><affiliation wicri:level="2"><nlm:affiliation>Department of Ecology and Evolutionary Biology, The University of Arizona, 1041 E. Lowell St., Tucson, AZ 85721, USA. arnold@ag.arizona.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Ecology and Evolutionary Biology, The University of Arizona, 1041 E. Lowell St., Tucson, AZ 85721</wicri:regionArea><placeName><region type="state">Arizona</region></placeName></affiliation></author></analytic><series><title level="j">Journal of fungi (Basel, Switzerland)</title><idno type="eISSN">2309-608X</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Fungal endophytes are diverse and widespread symbionts that occur in the living tissues of all lineages of plants without causing evidence of disease. Culture-based and culture-free studies indicate that they often are abundant in the leaves of woody angiosperms, but only a few studies have visualized endophytic fungi in leaf tissues, and the process through which most endophytes colonize leaves has not been studied thoroughly. We inoculated leaf discs and the living leaves of a model woody angiosperm, <i>Populus trichocarpa</i>, which has endophytes that represent three distantly-related genera (<i>Cladosporium</i>, <i>Penicillium</i>, and <i>Trichoderma</i>). We used scanning electron microscopy and light microscopy to evaluate the timeline and processes by which they colonize leaf tissue. Under laboratory conditions with high humidity, conidia germinated on leaf discs to yield hyphae that grew epiphytically and incidentally entered stomata, but did not grow in a directed fashion toward stomatal openings. No cuticular penetration was observed. The endophytes readily colonized the interiors of leaf discs that were detached from living leaves, and could be visualized within discs with light microscopy. Although they were difficult to visualize within the interior of living leaves following in vivo inoculations, standard methods for isolating foliar endophytes confirmed their presence.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">29772709</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>01</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2309-608X</ISSN><JournalIssue CitedMedium="Internet"><Volume>4</Volume><Issue>2</Issue><PubDate><Year>2018</Year><Month>May</Month><Day>16</Day></PubDate></JournalIssue><Title>Journal of fungi (Basel, Switzerland)</Title><ISOAbbreviation>J Fungi (Basel)</ISOAbbreviation></Journal><ArticleTitle>Observations on the Early Establishment of Foliar Endophytic Fungi in Leaf Discs and Living Leaves of a Model Woody Angiosperm, Populus trichocarpa (Salicaceae).</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E58</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/jof4020058</ELocationID><Abstract><AbstractText>Fungal endophytes are diverse and widespread symbionts that occur in the living tissues of all lineages of plants without causing evidence of disease. Culture-based and culture-free studies indicate that they often are abundant in the leaves of woody angiosperms, but only a few studies have visualized endophytic fungi in leaf tissues, and the process through which most endophytes colonize leaves has not been studied thoroughly. We inoculated leaf discs and the living leaves of a model woody angiosperm, <i>Populus trichocarpa</i>, which has endophytes that represent three distantly-related genera (<i>Cladosporium</i>, <i>Penicillium</i>, and <i>Trichoderma</i>). We used scanning electron microscopy and light microscopy to evaluate the timeline and processes by which they colonize leaf tissue. Under laboratory conditions with high humidity, conidia germinated on leaf discs to yield hyphae that grew epiphytically and incidentally entered stomata, but did not grow in a directed fashion toward stomatal openings. No cuticular penetration was observed. The endophytes readily colonized the interiors of leaf discs that were detached from living leaves, and could be visualized within discs with light microscopy. Although they were difficult to visualize within the interior of living leaves following in vivo inoculations, standard methods for isolating foliar endophytes confirmed their presence.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Yu-Ling</ForeName><Initials>YL</Initials><AffiliationInfo><Affiliation>School of Plant Sciences, The University of Arizona, 1140 E. South Campus Drive, Tucson, AZ 85721, USA. ylhuang@nmns.edu.tw.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>National Museum of Natural Science, 1 Guancian Rd., Taichung 40453, Taiwan. ylhuang@nmns.edu.tw.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zimmerman</LastName><ForeName>Naupaka B</ForeName><Initials>NB</Initials><Identifier Source="ORCID">0000-0003-2168-6390</Identifier><AffiliationInfo><Affiliation>School of Plant Sciences, The University of Arizona, 1140 E. South Campus Drive, Tucson, AZ 85721, USA. nzimmerman@usfca.edu.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Biology, University of San Francisco, Harney 219C, 2130 Fulton Street, San Francisco, CA 94117, USA. nzimmerman@usfca.edu.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Arnold</LastName><ForeName>A Elizabeth</ForeName><Initials>AE</Initials><AffiliationInfo><Affiliation>School of Plant Sciences, The University of Arizona, 1140 E. South Campus Drive, Tucson, AZ 85721, USA. arnold@ag.arizona.edu.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Ecology and Evolutionary Biology, The University of Arizona, 1041 E. 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Elizabeth" last="Arnold">A Elizabeth Arnold</name><name sortKey="Arnold, A Elizabeth" sort="Arnold, A Elizabeth" uniqKey="Arnold A" first="A Elizabeth" last="Arnold">A Elizabeth Arnold</name><name sortKey="Zimmerman, Naupaka B" sort="Zimmerman, Naupaka B" uniqKey="Zimmerman N" first="Naupaka B" last="Zimmerman">Naupaka B. Zimmerman</name><name sortKey="Zimmerman, Naupaka B" sort="Zimmerman, Naupaka B" uniqKey="Zimmerman N" first="Naupaka B" last="Zimmerman">Naupaka B. Zimmerman</name></country><country name="Taïwan"><noRegion><name sortKey="Huang, Yu Ling" sort="Huang, Yu Ling" uniqKey="Huang Y" first="Yu-Ling" last="Huang">Yu-Ling Huang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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